Resources

Antibody Information and Protocols

Antibody Lists

• Current Antibody List (Set 152-Present): Expanded Antibody List
  
• Previous Antibody List (up to Set 151): Standard Antibody List 
  

Previous Antibody Lists contained incorrect or uncommon gene names that caused problems during RPPA data
analysis. View the list of Updated Gene Names in the current antibody list for the latest information. 

Additional Antibody Charges

View the Services page for complete pricing information.

• Antibody Probing
  • Investigator-supplied antibody (at least 50 μl) previously validated and probed by Core: $300/ab and sample set (+ overhead fee for external out-of-network and non-academic institutions) 
•  Antibody Validation 
  • Investigator-supplied antibody (at least 100 μl) never validated and probed by Core: $750/ab and sample set (+ overhead fee for external out-of-network and non-academic institutions)*
  • Please provide at least 120 μl of your positive and negative cell lysates, full length Western blot results, and densitometer reading of each band if possible.

*Charge includes validation testing of your antibody as well as probing your RPPA sample set 

Antibody Validation for RPPA 

Our core extensively validates antibodies applicable to RPPA analysis. 

• If available, correlation between RPPA and mass spectrometry or RPPA and RNA expression with a Spearman correlation coefficient greater than 0.5. If correlation is less than 0.5, validation will be determined by Western blot.
  
• A single or dominant band on Western blotting is required and dynamic range and specificity is determined using peptides, phosphopeptides, growth factors, inhibitors, RNAi, and/or cells with wide levels of expression including 330 cell lines under multiple conditions on a single array.
• A Pearson correlation coefficient between RPPA and Western blotting of greater than 0.7 is required
• Reproducible results within a slide and between slides are critical. 

Example of antibody validation data