Resources, Technologies & Platforms
Instrumentation
- Leica Bond RXm™ autostainer
- Aperio AT Turbo™ scanner
- Aperio Image Scope™ analysis software (Leica Biosystems)
Expert pathology evaluation and data interpretation
- Scanning using the Leica Aperio AT Turbo slide scanning system
- Pathology evaluation and quality control
- Data interpretation
- Digital images availability
Automated immunohistochemical analysis
- Immunohistochemistry (IHC) analysis and quality control (Leica Bond RXm system)
- Manual Immunohistochemistry (IHC) is performed on an as-needed basis for antibody validation and unique markers
- Leica AT Turbo digital scanning system
- Digital image IHC analysis using Aperio ImageScope software or manually by expert pathologists
- Data consolidation using Aperio ImageScope software and H-Score
- Final report in Excel File format
Digital image analysis
The Aperio AT Turbo slide scanning system and ImageScope provides a comprehensive quantitative analysis of the biomarker of interest in the tissue sample. The tissue area of interest is examined by an expert team of pathologists and algorithm(s) are designed by the TMP-DL team with expertise in digital image analysis.
Antibody validation
Biomarker(s) expression in tumors and the microenvironment, as well as the immune infiltrates in tumors and skin toxicities, can be performed using singlet IHC assay, both in human and murine specimens.
Optimized biomarkers: human and mouse
Cell signal transduction: PTEN, Phospho-ERK, BRAF, etc.
Immune checkpoint signaling: e.g., PD-1, Ox-40, TIM3, etc. PD-L1, tumor infiltrating lymphocytes (e.g., CD4, CD8, T-regs, etc.) within the tumor microenvironment.
Antibody validation and optimization can be performed upon request by the Principal Investigator. All antibodies are optimized for IHC by examination of positive and negative controls, according to published research and after expert discussion with the Principal investigator.