MPL Mutational Analysis
Indication
Approximately 50% of essential thrombocythemia (ET) and primary myelofibrosis (PMF) have the p.V617F activating mutation in JAK2; a smaller proportion of ET cases (5-10%) have an activating mutation in codon 515 of the thrombopoietin receptor, MPL. This assay can also be used to track levels of residual disease in MPL-mutated tumors following treatment.
Methodology
PCR-based pyrosequencing of DNA is performed to examine mutational status of codon 515 of MPL. The level of mutated/unmutated PCR product is reported.
Test Parameters
Tumor clone must comprise at least 5% of the cells in the sample for accurate assessment. Levels are quantitative with approximately 5-10% variability.
Turnaround Time
10 days
Sample Requirements
- 10 ml peripheral blood, 2-5mL bone marrow aspirate, 5ug purified DNA
or
- 4-10 unstained tissue sections on glass slides along with 1 H&E-stained section.
Please provide a copy of the corresponding pathology report.
CPT Codes
81402
Additional charges may apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.