Advanced Microscopy Core
Collene Jeter, Ph.D.
Director
- Research Resources
- Core Facilities and Services
- Advanced Microscopy Core
Overview
Optical microscopy is vital to dissect the cellular and molecular basis of oncogenesis and pathophysiological processes in their native context in situ. Multispectral 3D and 4D (time lapse) fluorescence microscopy permits simultaneous imaging of numerous molecular and cellular biomarkers non-destructively to assess structural and behavioral aspects of cells, tissues and organs. Research impacts include studies of cellular protein distribution, cell fate, tumor heterogeneity, drug delivery and tumoricidal activity, DNA damage repair, development, neurodegeneration, cell migration/invasion, angiogenesis and cancer-stroma interactions, among others.
Getting Started
Equipment reservations are available on iLab:
https://mdanderson.ilabsolutions.com/sc/4846/mdacc-advanced-microscopy-core
Appointment instructions for assisted imaging or training:
- Begin on the Schedule Equipment tab
- Click on the View Schedule button for the equipment of interest
- Click and drag the desired time frame
- Select training or assisted imaging
- Core staff will approve your reservation, or will contact you to reschedule
- Contact staff for specimen hand-over and physical access.
Equipment booking instructions for approved trained users:
- Begin on the Schedule Equipment tab
- Click on the View Schedule button for the equipment of interest
- Click and drag the desired time frame and confirm your Self Use appointment
For assistance, training and access please contact:
- 3SCR: AMC Director Dr. Collene Jeter or Han Yang
- 2SCR: AMC Manager Anna Zal
Approved trained users may use equipment 24/7 subject to booking.
Cancelations within 24 hours of appointment, no shows or appointments less than 50% of time booked are subject to a 50% of the scheduled appointment cost. Please contact core staff for more information or to request an exception.
Impact
The Advanced Microscopy Core provides microscopy services to more than 30 departments at MD Anderson, comprising thousands of hours of high resolution and multispectral fluorescence microscopy annually for users from divisions including Basic Research, Cancer Medicine, Diagnostic Imaging, Surgery, Internal Medicine Pediatrics and Radiation Oncology, among others.
Acknowledgment
Publications should cite the Advanced Microscopy Core in the Acknowledgment section. Please let us know when your paper gets accepted.
Pilot Experiments
Pilot experiments are currently accepted, at a discounted rate, for intravital and lightsheet imaging. Sample submissions are highly encouraged.
Zeiss LSM880 Laser Scanning Confocal Microscope with Airyscan Detector
Analysis of actin cytoskeleton. Phalloidin-AF488 staining of the actin (green) and DAPI staining of cell nuclei (blue).
Amelie Albrecht/Snow Shen
Leica Confocal Laser Scanning Microscope
Peyer's patch in mouse intestine, fresh tissue, 3D projection. Treg cells (GFP, green), tissue surface (Cy5, red).
M. Anna Zal/Tomasz Zal
OMX Blaze V4 SIM Super-Resolution Microscope
T cells interacting with VCAM-1 on glass coverslip; actin (green), integrin α4β1 (blue), B-Raf (red).
Brown et al., JBC, 2014