Microinjection techniques performed at the GEMF
Genetically Engineered Mouse Facility
Director
Fabien Delerue, Ph.D.
- Research Resources
- Core Facilities and Services
- Genetically Engineered Mouse Facility
Overview
The Genetically Engineered Mouse Facility (GEMF) is a platform that generates customized rodent models for medical research purposes. We use cutting-edge technologies (including engineered endonucleases such as TALEN and CRISPR) for genome editing and aim at developing sophisticated animal models for the study of human diseases, particularly cancer. Learn more about GEMF.
Services offered by GEMF include:
- gene modification (e.g., pronuclear injection of zygotes using plasmids and BAC, targeted transgenesis by ES cells injection of blastocysts or injection of engineered nucleases in zygotes)
- fusion/aggregation chimeras, blastocyst complementation
- assisted reproductive technologies (e.g., in-vitro fertilization, cryopreservation, rederivation, etc.)
- analysis and quality control (e.g., genotyping, long and short reads sequencing, background characterization, SNP analysis etc.)
We also provide expert advice on design and implementation of projects, streamlining of the workflow and management of colonies.
Getting Started
It is critical that you contact the director of GEMF, Fabien Delerue, Ph.D., at fdelerue@mdanderson.org to discuss your project and agree on the editing strategy before submitting a request. We may not accept reagents directly from principal investigators and therefore highly advise you to get in touch before ordering any reagent.
Impact
Over the years our core delivered hundreds of valuable models to help develop breakthroughs and new therapeutic avenues. Most of these models resulted in high impact research and top tiered publications.
Acknowledgment
Please use the following text when acknowledging the core: "The Genetically Engineered Mouse Facility is supported in part by The University of Texas MD Anderson Cancer Center and the Cancer Center Support Grant (CCSG- NIH) P30CA016672."
Go to the Acknowledgment page to see guidelines on whether the efforts of core personnel merit authorship.
Contact
MD Anderson Cancer Center
Mitchell Basic Sciences Research Building (BSRB)
Access through Floor 2, J Elevator
Fabien Delerue, Ph.D.
Director, GEMF
Associate Professor
Department of Genetics
S2.8020
713-563-5827
fdelerue@mdanderson.org
New Services
As part of our quality control (QC), we now offer various sequencing solutions to assess the editing outcomes. These include Sanger sequencing, next generation sequencing (NGS) and nanopore long read sequencing (LRS).
News
Fabien Delerue, Ph.D., is awarded the 2023 International Society for Transgenic Technologies (ISTT) 3Rs Award
The 3Rs Award recognizes outstanding achievements by a scientist or a research team who keep the field of transgenic technologies vibrant with new methodologies and improvements in strict accordance with the 3Rs principles of humane experimental technique. Dr. Delerue received the 2023 ISTT 3Rs Award for “Long Read Sequencing reveals high frequency of bystander mutations following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes.”
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