Resources
Consultations regarding experiment design and protocols are available upon request.
Spectra Viewers for Planning Labeling with Fluorophores
Zeiss Confocal and Lightsheet ZEN Software
IMARIS tutorials
IF Staining for Confocal and Multiphoton Microscopy
Life-Canvas Clearing and Immunofluorescence Staining Protocols
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Outline
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Perfusion
To reduce autofluorescence or nonspecific antibody labeling; recommended for all tissues and organs.
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SHIELD Epoxide Crosslinking
Tissue preservation protocols and reagents required to preserve tissue architecture and epitopes before tissue clearing
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Passive Clearing
Detergent-mediated delipidation by diffusion; recommended for smaller tissues and organs.
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SmartClear Pro II Active Clearing
Stochastic electrotransport to more rapidly drive detergent-based delipidation; recommended for large organs.
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SmartLabel Active Staining
Stochastic electrotransport for rapid, homogeneous whole organ immunofluorescence staining.
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Tissue Specific Delipidation Advanced Protocols
Recommendations for fatty (fDISCO), heme-rich (e.g., liver) and other difficult samples.
Image Quantification Tutorials
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