Gallery
OMX Blaze V4 SIM Super-Resolution Microscope
T cells interacting with VCAM-1 on glass coverslip; actin (green), integrin α4β1 (blue), B-Raf (red).
Brown et al., JBC, 2014
Zeiss LSM880 Laser Scanning Confocal Microscope with Airyscan Detector
High resolution confocal imaging of mitochondria. MitoTracker-CMX-Ros staining (red) to visualize and assess mitochondria surrounding cell nuclei (DAPI; blue).
Collene Jeter
Zeiss LSM880 Laser Scanning Confocal Microscope with Airyscan Detector
Lysosomes in cancer cells. Lysosomes were stained with antibodies against LAMP1 (green), dynein motor protein LC8 (red) and DAPI (blue). Imaris image analysis was applied downstream to quantify lysosomes and determine subcellular distribution.
Collene Jeter/Shawn Bratton
Zeiss LSM880 Laser Scanning Confocal Microscope with 355 nm OPSL Laser for UVA Microirradiation
Laser microirradiation to track DNA damage in real time in situ. Snapshot of time lapse imaging of GFP-tagged DNA damage repair factor recruited in seconds to site of DNA damage following UVA laser microirradiation.
Collene Jeter/Leilei Shi/Mark Bedford
Leica SP8 DIVE Multiphoton Microscope
Astrocytosis and Tau plaques in transgenic Alzheimer’s neurodegenerative disease mouse model. Hippocampus vibratome sectioning and immunofluorescence staining shows GFAP+ astrocytes (red) upregulation coincident with Tau plaque formation (green).
Collene Jeter/Hong Seok Shim/Ronald DePinho
Leica SP8 DIVE Multiphoton Microscope
Pancreatic tissue regeneration in transgenic mTmG mouse. Stromal (red), epithelial (green) cells and nuclei (blue) participating in tissue regeneration following insult.
Collene Jeter/Rutvi Shah/I Lin Ho/Andrea Viale
OMX Blaze V4 SIM Super-Resolution Microscope
Mitochondria analysis in OCI-AML3 cells expressing GFP in mitochondria (green) and stained with DAPI (blue). Left: fluorescence intensity (scale bar 5μm), right: isosurface rendering using Imaris software.
Bhattacharya et al., Mol. Cancer Research, 2023
OMX Blaze V4 SIM Super-Resolution Microscope
Mitochondria analysis in Par. or MR MOLM-13 and Kasumi-1 cells stained with MitoTracker (magenta), TOM20 (red), MFN2 (green), DNA (blue).
Glytsou et al., Cancer Discovery, 2023
Leica SP8 Laser Scanning Confocal Microscope and Imaris Software
Redox mitochondrial reporter (roGFP)-expressing cells. Individual mitochondria objects were identified using Imaris. Color heatmap represents the ratio of the mean fluorescence intensity (MFI) at 405 nm excitation to 488 nm excitation (oxidized/reduced). Scale bar: 7μm.
Danesh et al., Nature Communications (in press).
Leica SP8 Laser Scanning Confocal Microscope
Epithelial pancreatic organoids. Left: fluorescent staining with pS10-H3 (green) and DAPI (blue). Right: Imaris 3D object identification.
M. Anna Zal/Francesca Citron/Ronald DePinho
Leica SP8 Laser Scanning Confocal Microscope (left), OMX Blaze V4 SIM Super-Resolution Microscope (right)
DNA fiber assay. DNA stained with anti-CldU and anti-IdU. Scale bar: 5 μm.
M. Anna Zal/Francesca Citron/Ronald DePinho
Leica SP8 Laser Scanning Confocal Microscope
3D projection of clarified mouse skin section; hair follicles (gray), stem cells (red), T-reg cells (green), nuclei (blue).
Tomasz Zal/Yejing Ge
Leica SP8 Laser Scanning Confocal Microscope
Analysis of microglia and T cells distribution around hypoxic zones in GL261 murine glioblastoma. Tissue section stained for microglia (Tmem119, red), T cells (CD3, white) and hypoxia (Pimonidazole, green).
Ivana William/Michael Curran
Leica SP8 Laser Scanning Confocal Microscope
Reflection microscopy of silver impregnated pyramidal cortical neurons in aged Alzheimer’s disease mouse brain.
Hong Seok Shim/Tomasz Zal/Ronald DePinho