Publications
2024
Carvajal-Maldonado D, Li Y, Returan M, Averill AM, Doublié S, Wood RD. Dynamic stem-loop extension by Pol θ and templated insertion during DNA repair. J Biol Chem. 2024 Jul;300(7):107461.
Carvajal-Maldonado D, Zahn K, Jensen R, Wood RD, Doublié S. Human DNA polymerase θ does not harbor intrinsic nuclease activity. Mol Cell. 2024 Apr 18;84(8):1394-1395.
Fijen C, Drogalis Beckham L, Terino D, Li Y, Ramsden DA, Wood RD, Doublié S, Rothenberg E. Sequential requirements for distinct Polθ domains during theta-mediated end joining. Mol Cell. 2024 Apr 18;84(8):1460-1474.e6.
2023
Stroik S, Carvajal-Garcia J, Gupta D, Edwards A, Luthman A, Wyatt DW, Dannenberg RL, Feng W, Kunkel TA, Gupta GP, Hedglin M, Wood R, Doublié S, Rothenberg E, Ramsden DA. Stepwise requirements for polymerases δ and θ in theta-mediated end joining. Nature. 2023 Nov;623(7988):836-841.
2022
Wood RD, Doublié S. Genome Protection by DNA Polymerase θ. Annu Rev Genet. 2022 Nov 30;56:207-228.
Vanson S, Li Y, Wood RD, Doublié S. Probing the structure and function of polymerase θ helicase-like domain. DNA Repair (Amst). 2022 Aug;116:103358. PMID: 35753097
Carvajal-Maldonado D, Drogalis Beckham L, Wood RD, Doublié S. When DNA Polymerases Multitask: Functions Beyond Nucleotidyl Transfer. Front Mol Biosci. 2022 Jan 7;8:815845. PMID:35071329
2021
Ben Yamin B, Ahmed-Seghir S, Tomida J, Despras E, Pouvelle C, Yurchenko A, Goulas J, Corre R, Delacour Q, Droin N, Dessen P, Goidin D, Lange SS, Bhetawal S, Mitjavila-Garcia MT, Baldacci G, Nikolaev S, Cadoret JC, Wood RD, Kannouche PL. DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability. EMBO J. 2021 Nov 2;40(21):e104543. doi: 10.15252/embj.2020104543. PMID:34533226
Llorens-Agost M, Ensminger M, Le HP, Gawai A, Liu J, Cruz-García A, Bhetawal S, Wood RD, Heyer WD, Löbrich M. POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis. Nat Cell Biol. 2021 Oct;23(10):1095-1104. doi: 10.1038/s41556-021-00764-0. PMID:34616022
Carvajal-Maldonado D, Wood RD. Regulating Polθ in Breast Cancer. Cancer Res. 2021 Mar 15;81(6):1441-1442. (Commentary on Prodhomme et al, EMT transcription factor ZEB1 represses the mutagenic Polθ-mediated end-joining pathway in breast cancers.) PMID:33723002
Martin, SK, Tomida J, Wood RD. Disruption of DNA polymerase ζ (zeta) engages an innate immune response. Cell Reports. 2021 Feb 23; 34(8):108775. PMID:33626348
2020
Wang R, Lenoir WF, Wang C, Su D, McLaughlin M, Hu Q, Shen X, Tian Y, Klages-Mundt N, Lynn E, Wood RD, Chen J, Hart T, Li L. DNA polymerase ι compensates for Fanconi anemia pathway deficiency by countering DNA replication stress. Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33436-33445. PMID:33376220
Hwang T, Reh S, Dunbayev Y, Zhong Y, Takata Y, Shen J, McBride K, Murnane J, Bhak J, Lee S, Wood R, Takata K. Defining the mutation signature of DNA polymerase θ in cancer genomes. NAR Cancer. 2020 Sep;2(3):zcaa017. PMID:32885167
Carvajal-Garcia J, Cho JE, Carvajal-Garcia P, Feng W, Wood RD, Sekelsky J, Gupta GP, Roberts SA, Ramsden DA. Mechanistic basis for microhomology identification and genome scarring by polymerase theta. Proc Natl Acad Sci U S A. 2020 Apr 14;117(15):8476-8485. PMID:32234782
2019
Liu X, Jiang Y, Takata KI, Nowak B, Liu C, Wood RD, Hittelman WN, Plunkett W. CNDAC-induced DNA double strand breaks cause aberrant mitosis prior to cell death. Mol Cancer Ther. 2019 Dec;18(12):2283-2295. PMID:31501277
Feng W, Simpson DA, Carvajal-Garcia J, Price BA, Kumar RJ, Mose LE, Wood RD, Rashid N, Purvis JE, Parker JS, Ramsden DA, Gupta GP. Genetic determinants of cellular addiction to DNA polymerase theta. Nat Commun. 2019 Sep 19;10(1):4286. PMID:31537809
Martin SK, Wood RD. DNA polymerase ζ in DNA replication and repair. Nucleic Acids Res. 2019 Sep 19;47(16):8348-8361. PMID:31410467
2018
Lange SS, Bhetawal S, Reh S, Powell KL, Kusewitt D, Wood RD. DNA polymerase ζ deficiency causes impaired wound healing and stress-induced skin pigmentation. Life Science Alliance. 2018 June 29; 1(3):e201800048. PMID:30046772
Tomida J, Takata KI, Bhetawal S, Person MD, Chao HP, Tang DG, Wood RD. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 2018 Jun 15;37(12):e99543. PMID:29789392
Wood RD. Fifty years since DNA repair was linked to cancer. Nature. 2018 May;557(7707):648-649. PMID:29844546 (Commentary)
Wood RD, Manandhar M, Lowery M, Boulware KS, Lin K, Lu Y. Response to "XPA is primarily cytoplasmic but is transported into the nucleus upon UV damage". DNA Repair (Amst). 2018 Feb;62:30-31. PMID:29337160
2017
Manandhar M, Lowery MG, Boulware KS, Lin KH, Lu Y, Wood RD. Transcriptional consequences of XPA disruption in human cell lines. DNA Repair (Amst). 2017 Sep;57:76-90. PMID:28704716
Malaby AW, Martin SK, Wood RD, Doublié S. Expression and Structural Analyses of Human DNA Polymerase θ (POLQ). Methods Enzymol. 2017;592:103-121. PMID:28668117
Takata KI, Reh S, Yousefzadeh MJ, Zelazowski MJ, Bhetawal S, Trono D, Lowery MG, Sandoval M, Takata Y, Lu Y, Lin K, Shen J, Kusewitt DF, McBride KM, Cole F, Wood RD. Analysis of DNA polymerase ν function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet. 2017 Jun1;13(6):e1006818. PMID:28570559
Ishiai M, Sato K, Tomida J, Kitao H, Kurumizaka H, Takata M. Activation of the FA pathway mediated by phosphorylation and ubiquitination. Mutat Res. 2017 Oct;803-805:89-95. Review. PMID:28552166
2016
Lin YC, Owen N, Minko IG, Lange SS, Li L, Stone MP, Wood RD, McCullough AK, Lloyd RS. DNA polymerase ζ limits chromosomal damage and promotes cell survival following aflatoxin exposure. Proc Natl Acad Sci U S A. 2016 Nov 29;113(48):13774-13779. PMID:27849610
Wyatt DW, Feng W, Conlin MP, Yousefzadeh MJ, Roberts SA, Mieczkowski P, Wood RD, Gupta GP, Ramsden DA. Essential Roles for Polymerase θ-Mediated End Joining in the Repair of Chromosome Breaks. Mol Cell. 2016 Aug 18;63(4):662-73. PMID:27453047
Wood RD, Doublié S. DNA polymerase θ (POLQ), double-strand break repair, and cancer. DNA Repair (Amst). 2016 Aug;44:22-32. PMID:27264557
Schibler A, Koutelou E, Tomida J, Wilson-Pham M, Wang L, Lu Y, Cabrera AP, Chosed RJ, Li W, Li B, Shi X, Wood RD, Dent SY. Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2. Genes Dev. 2016 May 15;30(10):1187-97. PMID:27198228
Lange SS, Tomida J, Boulware KS, Bhetawal S, Wood RD. The Polymerase Activity of Mammalian DNA Pol ζ Is Specifically Required for Cell and Embryonic Viability. PLoS Genet. 2016 Jan 4;12(1):e1005759. PMID:26727495
2015
Perez CJ, Mecklenburg L, Jaubert J, Martinez-Santamaria L, Iritani BM, Espejo A, Napoli E, Song G, Del Rio M, DiGiovanni J, Giulivi C, Bedford MT, Dent SY, Wood RD, Kusewitt DF, Guenet JL, Conti CJ, Benavides F. Increased Susceptibility to Skin Carcinogenesis Associated with a Spontaneous Mouse Mutation in the Palmitoyl Transferase Zdhhc13 gene. J Invest Dermatol, 2015 Dec 135(12):3133-43. PMID:26288350
Takata K, Tomida J, Reh S, Swanhart LM, Takata M, Hukriede NA, Wood RD. Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN). J Biol Chem. 2015 Oct 2;290(40):24278-93. PMID:26269593
Manandhar M, Boulware KS, Wood RD. The ERCC1 and ERCC4 (XPF) genes and gene products. Gene. 2015 Sep 15;569(2):153-61. PMID:26074087
Zahn KE, Averill AM, Aller P, Wood RD, Doublié S. Human DNA polymerase θ grasps the primer terminus to mediate DNA repair. Nat Struct Mol Biol. 2015 Apr;22(4):304-11. PMID:25775267
Tomida J, Takata K, Lange SS, Schibler AC, Yousefzadeh MJ, Bhetawal S, Dent SY, Wood RD. REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ. Nucleic Acids Res. 2015 Jan;43(2):1000-11. PMID:25567983
2014
Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, Bylund GO, Doublié S, Johansson E, Ramsden DA, McBride KM, Wood RD. Mechanism of suppression of chromosomal instability by DNA polymerase POLQ. PLoS Genet. 2014 Oct 2;10(10):e1004654. PMID: 25275444
Unno J, Itaya A, Taoka M, Sato K, Tomida J, Sakai W, Sugasawa K, Ishiai M, Ikura T, Isobe T, Kurumizaka H, Takata M. FANCD2 binds CtIP and regulates DNA-end resection during DNA interstrand crosslink repair. Cell Rep. 2014 May 22;7(4):1039-47. PMID: 24794430
Wood RD, Lange SS. Breakthrough for a DNA break-preventer. Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):2864-5. PMID: 24532659 (Commentary)
2013
Wood RD. DNA damage tolerance and a web of connections with DNA repair at Yale. Yale J Biol Med. 2013 Dec 13;86(4):507-16. PMID:24348215
Takata K, Reh S, Tomida J, Person MD, Wood RD. Human DNA helicase HELQ participates in DNA interstrand crosslink tolerance with ATR and RAD51 paralogs. Nature Communications. 2013 Sep 4;4:2338. PMID:24005565
Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, Masuda Y, Matsuda S, Adachi J, Kobayashi M, Meetei AR, Maehara Y, Yamamoto K, Kamiya K, Matsuura A, Matsuda T, Ikura T, Ishiai M, Takata M. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res. 2013 Aug;41(14):6930-41. PMID:23723247
Lange SS, Bedford E, Reh S, Wittschieben JP, Carbajal S, Kusewitt DF, DiGiovanni J, Wood RD. Dual role for mammalian DNA polymerase zeta in maintaining genome stability and proliferative responses. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E687-96. PMID:23386725
Yousefzadeh MJ, Wood RD. DNA polymerase POLQ and cellular defense against DNA damage. DNA Repair. 2013 Jan 1;12(1):1-9. PMID:23219161
2012
Christov PP, Yamanaka K, Choi JY, Takata K, Wood RD, Guengerich FP, Lloyd RS, Rizzo CJ. Replication of the 2,6-Diamino-4-hydroxy-N(5)-(methyl)-formamidopyrimidine (MeFapy-dGuo) Adduct by Eukaryotic DNA Polymerases. Chem Res Toxicol. 2012 Aug 20;25(8):1652-61. PMID:22721435
Lange SS, Wittschieben JP, Wood RD. DNA polymerase zeta is required for proliferation of normal mammalian cells. Nucleic Acids Res. 2012 May;40(10):4473-82. PMID:22319213
Shigechi T, Tomida J, Sato K, Kobayashi M, Eykelenboom JK, Pessina F, Zhang Y, Uchida E, Ishiai M, Lowndes NF, Yamamoto K, Kurumizaka H, Maehara Y, Takata M. ATR-ATRIP kinase complex triggers activation of the Fanconi anemia DNA repair pathway. Cancer Res. 2012 Mar 1;72(5):1149-56. PMID: 22258451
Earlier Publications
2011 Publications
Lange SS, Takata K, Wood RD. DNA polymerases and cancer. Nature Reviews Cancer. 2011 Feb;11(2):96-110. PMID:21258395
Hogg M, Seki M, Wood RD, Doublié S, Wallace SS. Lesion bypass activity of DNA polymerase theta (POLQ) is an intrinsic property of the pol domain and depends on unique sequence inserts. J Mol Biol. 2011 Jan 21;405(3):642-52. PMID:21050863
2010 Publications
Lemée F, Bergoglio V, Fernandez-Vidal A, Machado-Silva A, Pillaire M-J, Bieth A, Gentil C, Baker L, Martin A-L, Leduc C, Lam E, Magdeleine E, Filleron T, Oumouhou N, Jordan L, Kaina B, Seki M, Grimal F, Lacroix-Triki M, Thompson A, Roche H, Bourdon J-C, Wood RD, Hoffmann J-S, Cazaux C. DNA polymerase theta up-regulation is associated with poor survival in breast cancer, perturbs DNA replication and promotes genetic instability. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13390-5. PMID:20624954
Wood RD. Mammalian nucleotide excision repair proteins and interstrand crosslink repair. Environ Mol Mutagen. 2010 Jul;51(6):520-6. PMID:20658645
Takata K, Arana ME, Seki M, Kunkel TA, Wood RD. Evolutionary conservation of residues in vertebrate DNA polymerase N conferring low fidelity and bypass activity. Nucleic Acids Res. 2010 Jun;38(10):3233-44. PMID:20144948
Wittschieben JP, Patil V, Glushets V, Robinson LJ, Kusewitt DF, Wood RD. Loss of DNA polymerase zeta enhances spontaneous tumorigenesis. Cancer Res. 2010 Apr 1;70(7):2770-8. PMID:20215524
Yamanaka K, Minko IG, Takata K-i, Kolbanovskiy A, Kozekov ID, Wood RD, Rizzo CJ, Lloyd RS. Novel enzymatic function of DNA polymerase ν in translesion DNA synthesis past major groove DNA−peptide and DNA−DNA cross-links. Chem Res Toxicol. 2010 Mar 15;23(3):689-95. PMID:20102227
2009 Publications
Bhagwat NR, Roginskaya VY, Acquafondata MB, Dhir R, Wood RD, Niedernhofer LJ. Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue. Cancer Res. 2009 Sep 1;69(17):6831-8. PMID:19723666
Goff JP, Shields DS, Seki M, Choi S, Epperly MW, Dixon T, Wang H, Bakkenist CJ, Dertinger SD, Torous DK, Wittschieben J, Wood RD, Greenberger JS. Lack of DNA polymerase theta (POLQ) radiosensitizes bone marrow stromal cells in vitro and increases reticulocyte micronuclei after total-body irradiation. Radiat Res. 2009 Aug;172(2):165-74. PMID:19630521
Takata K, Wood RD. Bypass specialists operate together. EMBO J. 2009 Feb 18;28(4):313-4. PMID:19225445
2008 Publications
Köberle B, Roginskaya V, Zima KS, Masters JR, Wood RD. Elevation of XPA protein level in testis tumor cells without increasing resistance to cisplatin or UV radiation. Mol Carcinog. 2008 Aug;47(8):580-6. PMID:18240296
Arana ME, Seki M, Wood RD, Rogozin IB, Kunkel TA. Low-fidelity DNA synthesis by human DNA polymerase theta. Nucleic Acids Res. 2008 Jun;36(11):3847-56. PMID:18503084
Gan GN, Wittschieben JP, Wittschieben BØ, Wood RD. DNA polymerase zeta (pol ζ) in higher eukaryotes. Cell Res. 2008 Jan;18(1):174-83. PMID:18157155
Seki M, Wood RD. DNA polymerase theta (POLQ) can extend from mismatches and from bases opposite a (6-4) photoproduct. DNA Repair (Amst). 2008 Jan1;7(1):119-27. PMID:17920341
2007 Publications
Masuda K, Ouchida R, Hikida M, Kurosaki T, Yokoi M, Masutani C, Seki M, Wood RD, Hanaoka F, O-Wang J. DNA polymerases eta and theta function in the same genetic pathway to generate mutations at A/T during somatic hypermutation of Ig genes. J Biol Chem. 2007 Jun 15;282(24):17387-94.
Niedernhofer LJ, Bhagwat N, Wood RD. ERCC1 and non-small-cell lung cancer. N Engl J Med. 2007 Jun 14;356(24):2538-40; author reply 2540-1.
Friedberg EC, Wood RD. New insights into the combined Cockayne/xeroderma pigmentosum complex: human XPG protein can function in transcription factor stability. Mol Cell. 2007 Apr 27;26(2):162-4. Review.
Arana ME, Takata K, Garcia-Diaz M, Wood RD, Kunkel TA. A unique error signature for human DNA polymerase nu. DNA Repair (Amst). 2007 Feb 4;6(2):213-23.
2006 Publications
Yoshimura M, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde JM, Hochegger H, Okada T, Hiraoka M, Takeda S. Vertebrate POLQ and POL beta cooperate in base excision repair of oxidative DNA damage. Mol Cell. 2006 Oct 6;24(1):115-25.
Friedberg EC, Aguilera A, Gellert M, Hanawalt PC, Hays JB, Lehmann AR, Lindahl T, Lowndes N, Sarasin A, Wood RD. DNA repair: from molecular mechanism to human disease. DNA Repair (Amst). 2006 Aug 13;5(8):986-96.
Takata K, Shimizu T, Iwai S, Wood RD. Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol. J Biol Chem. 2006 Aug 18;281(33):23445-55.
Shivji MK, Moggs JG, Kuraoka I, Wood RD. Assaying for the dual incisions of nucleotide excision repair using DNA with a lesion at a specific site. Methods Mol Biol. 2006;314:435-56.
Biggerstaff M, Wood RD. Repair synthesis assay for nucleotide excision repair activity using fractionated cell extracts and UV-damaged plasmid DNA. Methods Mol Biol. 2006;314:417-34.
Köberle B, Roginskaya V, Wood RD. XPA protein as a limiting factor for nucleotide excision repair and UV sensitivity in human cells. DNA Repair (Amst). 2006 May 10;5(5):641-8.
Wittschieben JP, Reshmi SC, Gollin SM, Wood RD. Loss of DNA polymerase zeta causes chromosomal instability in mammalian cells. Cancer Res. 2006 Jan 1;66(1):134-42.
2005 Publications
Seki M, Gearhart PJ, Wood RD. DNA polymerases and somatic hypermutation of immunoglobulin genes. EMBO Rep. 2005 Dec;6(12):1143-8. Review.
Wittschieben BØ, Iwai S, Wood RD. DDB1-DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic/apyrimidinic sites, and compound lesions in DNA. J Biol Chem. 2005 Dec 2;280(48):39982-9.
Clarkson SG, Wood RD. Polymorphisms in the human XPD (ERCC2) gene, DNA repair capacity and cancer susceptibility: an appraisal. DNA Repair (Amst). 2005 Sep 28;4(10):1068-74. Review.
Wood RD, Mitchell M, Lindahl T. Human DNA repair genes, 2005. Mutat Res. 2005 Sep 4;577(1-2):275-83. Review.
2004 Publications
Thorel F, Constantinou A, Dunand-Sauthier I, Nouspikel T, Lalle P, Raams A, Jaspers NG, Vermeulen W, Shivji MK, Wood RD, Clarkson SG. Definition of a short region of XPG necessary for TFIIH interaction and stable recruitment to sites of UV damage. Mol Cell Biol. 2004 Dec;24(24):10670-80.
Seki M, Masutani C, Yang LW, Schuffert A, Iwai S, Bahar I, Wood RD. High-efficiency bypass of DNA damage by human DNA polymerase Q. EMBO J. 2004 Nov 10;23(22):4484-94.
Welsh C, Day R, McGurk C, Masters JR, Wood RD, Köberle B. Reduced levels of XPA, ERCC1 and XPF DNA repair proteins in testis tumor cell lines. Int J Cancer. 2004 Jun 20;110(3):352-61.
2003 Publications
Seki M, Marini F, Wood RD. POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in human cells. Nucleic Acids Res. 2003 Nov 1;31(21):6117-26.
Wittschieben BØ, Wood RD. DDB complexities. DNA Repair (Amst). 2003 Sep 18;2(9):1065-9. Review.
Marini F, Kim N, Schuffert A, Wood RD. POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308. J Biol Chem. 2003 Aug 22;278(34):32014-9.
2002 Publications
Marini F and Wood RD. A human DNA helicase homologous to the DNA cross-link sensitivity protein Mus308. J Biol Chem. 2002 Mar 8;277(10):8716-23.