Resources

ZipSeq related materials:

• ZipSeq Reagent Setup and QC Rev D [updated 6/16/2023]
  This short guide takes you through the best practices I've found for generating the oligo-Ab conjugates described in our Nature Methods paper and how to QC them on test cells with flow cytometry. It also lays out how to test hybridization of strands, titrations and photouncaging 'binariness,' as well as strand sequences published and unpublished.
  
  
• ZipSeq Sectioning and Mounting
  This short guide takes you through how to section your tissue well, which is critical for the ZipSeq method. This document describes sectioning performed on lymph nodes as an example, but it works with PyMT tumors as well.